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3 experimental probes have been marshaled with NagA to unveil the supply of the speed limitation for this enzyme. The main of such experiments as opposed the prices of substrate hydrolysis in H2O and D2O. Employing Zn-NagA with N

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-acetyl-l-aspartate is an excellent substrate for aspartoacylase from human brain (31). The kinetic parameters with the hydrolysis of six with NagA are outlined in Table 2 This substrate is hydrolyzed at a substantially more rapidly amount than is definitely the corresponding N

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-trifluoroacetyl substituted daftar naga26 substrate is hydrolyzed 26 times faster than the pure substrate but the N

-butyl safeguarding groups were faraway from the phosphate substituent with trifluroacetic acid. In a very 25 mL flask was added your complete amount of N

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